BIO 354 Cell Bio Lab Exam
Imagine you are a scuba diver the size of a virus exploring huge algae cells. If you
wanted to see how the water molecules are split inside the thylakoids space, how many
membranes would you have to cross to get there?
3
You would go through the cell wall, to get the plasma membrane then you would be
inside the cytoplasm. From there you would swim to the chloroplast and cross the outer
and I. Er membranes to get the storms, where you'd see colorful stacks of thylakoids.
Where are the pigment molecules held in place?
By the photosystem, a protein complex.
Why do pigments absorb visible light and other molecules don't
Extended conjugated systems
Extended conjugated systems are alternating double bonds and/or atoms with a free
electron pair. Usually nitrogen and oxygen
Chloroplast are suspended in an isolation buffer. To be able to extract the pigments
from the chloroplast, we have to get rid of the first buffer first. What's the best way to
separate the chloroplast from the buffer
Centrifugation.
Inside the spinning centrifuge, the sample is separated according to the densities if the
particles
To elite all the pigment contained in the chloroplasts, what do you have to consider,
when choosing from different solvents?
Use both hydrophobic and hydrophilic solvents
What color is not absorbed by hexane according to the absorbance spectrum? What
kind of pigment is this?
Green, chlorophyll
What color does the water extract absorb
Green
Where does the light reaction take place?
thylakoid membrane