1. Multiple Choice: Which of the following is not a method for obtaining pure cultures of microorganisms? A. Streak plate method B. Pour plate method C. Agar diffusion method D. Spread plate method Correct answer: C. Agar diffusion method Rationale: The agar diffusion method is used for testing the susceptibility of microorganisms to antibiotics, not for obtaining pure cultures. 2. Fill-in-the-Blank: The process of transferring bacteria from one culture medium to another is known as ________. Correct answer: subculturing Rationale: Subculturing is important for maintaining the viability and purity of bacterial cultures. 3. True/False: The Gram stain is a differential staining technique used to classify bacteria based on the presence of a thick or thin cell wall. Correct answer: False Rationale: The Gram stain is a differential staining technique used to classify bacteria based on their cell wall composition.