(Some Questions denote their format. All questions are answered) 1. True/False: The accuracy of a micropipette is unaffected by the viscosity of the liquid being pipetted. - Answer: False. The viscosity of the liquid can affect the accuracy of a micropipette, as more viscous liquids may require more force to aspirate and dispense, potentially leading to inaccuracies. 2. Fill in the blank: When loading a sample into an agarose gel, it is important to avoid puncturing the gel and to insert the pipette tip just _______ the surface of the gel. - Answer: below. This prevents the sample from diffusing into the buffer and ensures it enters the well properly. 3. Multiple Choice: Which of the following factors does not affect the resolution of DNA fragments during electrophoresis? A) Agarose concentration B) Buffer composition C) Voltage applied D) Size of DNA fragments - Answer: C) Voltage applied. While voltage affects the speed of migration, it is not a factor in the resolution of DNA fragments. 4. Open-ended: Describe the steps you would take to ensure the accuracy and precision of your pipetting technique when preparing samples for electrophoresis. - Answer: Students should mention steps such as pre-wetting the pipette tip, calibrating the pipette regularly, using the correct pipette size for the volume, and practicing consistent pipetting angles and speeds. 5. True/False: Acrylamide gels are typically used for the separation of proteins due to their finer pore size compared to