1. What is the main difference between BSL-2 and BSL-3

in terms of laboratory design and practices?

a) BSL-3 requires negative air pressure and HEPA

filtration of exhaust air.

b) BSL-3 requires biosafety cabinets and personal

protective equipment for all procedures.

c) BSL-3 requires a separate changing room and shower

facility for personnel.

d) BSL-3 requires a double-door entry and an anteroom for

decontamination.

*Answer: a) BSL-3 requires negative air pressure and

HEPA filtration of exhaust air. Rationale: This is the main

feature that distinguishes BSL-3 from BSL-2, as it prevents

the escape of potentially infectious aerosols from the

laboratory.*

2. Which of the following agents is not typically handled in

a BSL-3 laboratory?

a) Mycobacterium tuberculosis

b) Bacillus anthracis

c) Ebola virus

d) Francisella tularensis

*Answer: c) Ebola virus. Rationale: Ebola virus is

classified as a Risk Group 4 agent, which requires BSL-4