MB (ASCP) EXAM 2023-2024 ATCUAL EXAM 300 QUESTIONS AND CORRECT DETAILED ANSWERS (100% VERIFIED ANSWERS) |ALREADY GRADED A+

MB (ASCP) EXAM 2023-2024 ATUAL EXAM 300

QUESTIONS AND CORRECT DETAILED

ANSWERS (100% VERIFIED ANSWERS)

|ALREADY GRADED A+

Consider the probe sequence, CTACCGTAATATTCGACCGT, to be

used in a hybridization procedure. What is the melting temperature, Tm,

of the sequence?

60°C

58°C

64°C

62°C - ANSWER- 58°C

This polymerase acts on DNA and produces Ribosomal RNA:

RNA Pol I

DNA Pol I

RNA Pol II

DNA Pol II - ANSWER- RNA Pol I

Sickle cell disease is an autosomal genetic disease due to a point

mutation in the beta-globin gene, where glutamic acid is substituted for

valine at the sixth codon of the gene, resulting in a faulty hemoglobin S

(Hb S). Sickle cell disease is one of many genetic diseases where a

single gene controls the expression of many phenotypic traits. The

phenomenon where a single gene controls the expression of many

phenotypic traits is best referred to as: - ANSWER- Pleiotrophy

What assay amplifies the target using a combination of a three-enzyme

system?

Branched DNA

TMA

PCR

NASBA

LCR - ANSWER- NASBA

In what part of a qPCR Amplification Plot do you take your

measurement? - ANSWER- Exponential phase

All of the following are liquid tumors, except:

Mantle cell lymphoma

Ewing sarcoma

Burkitt's lymphoma

Acute promyelocytic leukemia - ANSWER- Ewing sarcoma

A technologist uses the spectrophotometer to quantify the amount of

DNA extracted from a blood specimen diluted 1:30. The absorbance

reading at 260 nm was found to be 2.545. If the absorbance at 280 nm

gave a reading of 1.406, and if the the DNA extract was re-suspended in

0.800 mL of EDTA solution, the DNA yield is: - ANSWER- (2.545 *

50ng/mcl)= 127.25

(127.25ng/mcl * 0.30) = 38.175ng/mcl

38.175ng/mcl ( 0.800mL1000) =

3054 micrograms

According to the wobble hypothesis, a U in the 5' position of the anitcodon can pair with:

U or C

A or G

A, C or G

Only A - ANSWER- A or G

This polymerase acts on DNA and produces Messenger RNA: -

ANSWER- RNA Pol II

What assay amplifies the probe signal and the probe RNA concentration

increases if the target to be detected is present?

Branched DNA

TMA

PCR

NASBA

LCR

Qβ replicase - ANSWER- Qβ replicase

This restriction enzyme "digests and adds a methyl group from

Adenine":

Type I

Type II

Type III

DNase I - ANSWER- Type III

What increases the half-life of mRNA?

5' Methyl Cap

3' Methyl Cap

Poly-A tail

5' Methionine Cap - ANSWER- 5' Methyl Cap

A parent has an autosomal dominant disorder. What percent chance does

this parent have to pass down this affected gene to his/her child?

0%

25%

50%

75%

100% - ANSWER- 50%

The phrase "central dogma of molecular biology" refers to the flow of

genetic data in this manner:

DNA --> RNA --> Proteins

DNA -->Proteins --> Genes

RNA --> DNA --> Proteins

RNA --> Proteins --> DNA - ANSWER- DNA --> RNA --> Proteins

What drug is NOT metabolized by CYP2D6?

Codeine

Omeprazole

Warfarin

Escitalopram - ANSWER- Warfarin

You have sequenced a gene and observe the following:

Reference: atgctggcacgacaggtttcccgactgg

Sequenced: atgCctggcacgacaggtttcccgactgg

The mutation observed is a:

Frame-shift mutation

Insertion

Silent mutation

Non-conservative mutation - ANSWER- Frame-shift mutation

A parent has an autosomal recessive disorder. What percent chance does

this parent have to pass down this affected gene to his/her child?

0%

25%

50%

75%

100% - ANSWER- 100%