1. What are two types of bacteria and what the difference?  Gram Positive= thick peptidoglycan layer (purple) = Staph aureus are responsible with various infection  Gram Negative= thin peptidoglycan layer (pink)= klebsiella pneumoniae= less common form of pneumonia, streptococcus pneumoniae is most common  Lipid A in LPS present the cell wall of gram-negative bacteria and is a endotoxin. 2. What do you think could be challenging to apply evidence-based practice in nursing care? How can you overcome these when you become a nurse? Evidence-based practice (EBP) is the use of the best available research evidence, clinical expertise, and patient preferences to guide nursing decisions and interventions. EBP can improve the quality, safety, and effectiveness of nursing care, as well as reduce costs and enhance patient outcomes. However, there are some challenges to applying EBP in nursing care, such as: - Lack of time, resources, and skills to access, appraise, and implement research evidence - Resistance to change and low motivation among nurses and other health care providers - Inconsistency and variability in the availability and quality of research evidence - Conflicting or contradictory findings from different studies or sources - Ethical, legal, cultural, or organizational barriers that limit the applicability or feasibility of EBP To overcome these challenges, nurses need to: - Develop a culture of inquiry and curiosity that values EBP and supports lifelong learning - Seek out and participate in educational opportunities and mentorship programs that enhance EBP knowledge and skills - Collaborate with other nurses, researchers, and stakeholders to identify, prioritize, and address clinical problems and questions - Use reliable and user-friendly tools and resources to access, appraise, and synthesize research evidence - Apply critical thinking and clinical judgment to integrate research evidence with clinical expertise and patient preferences - Evaluate the outcomes and impact of EBP interventions and share the results and feedback with others - Advocate for organizational policies and practices that facilitate EBP implementation and dissemination 3. What is Sepsis? Sepsis is global problem, is a time critical emergency the mortality for patient with septic shock has shown increase 7.6% per hr delay after onset of hypotension in commencing antibiotics. Sepsis is a potentially life-threatening condition that occurs when the body's immune system overreacts to an infection, leading to widespread inflammation and organ dysfunction. It is often