AMCA-PTC Study Guide Objective #4--Specimen Collection Questions And Answers 2025/2026

1. When patients need more than one test performed,
requiring more than one tube to be filled, the same
multi-sample is used to fill all of the tubes. This
leads to potential of material from earlier tubes
needle, contamination, Clinical
and Laboratory
Standards Instiinto subsequent tubes. As a result,the tute
 (CLSI) has developed a standard order of draw for
multitube draws. This applies to both syringe samples
and multi-sample needle draws.
2. CLSI Order of Draw
(name colors in order)
1. Sterile Tube (blood cultures): top
2. Coagulation Tube: top
3. Serum Tube: top (glass or plastic tube)
4. SST (Serum Separator Tubes): Speckled Red/Gray,
Tiger top, or BD Hemoguard
5. PST (Plasma Separator Tubes): Light or Dark
top
6. EDTATube: top
7. Glycolytic Inhibitor Tube: top
3. It is very important that a , found in the workplace, be followed regarding specimen collection and
order-of-draw standards, even if they differ from the
order above.
4. The first and most important step in venipuncture
procedures is to properly the patient.
5. Erroneously drawing blood from the wrong patient
could lead to serious consequences, including incorrect or treatment of the intended patient, which
could result in injury or death, of the phlebotomist, and the potential for a lawsuit.
6. To reduce the risk of incorrect identification of a patient,two of the following three main identifiers should
be used before drawing a patient's blood:
Yellow, Light Blue,
Red, Gold, Green,
Lavender, Gray
Procedure Manual
identify
diagnosis, termination
name, DOB,