1. A case study in the Amazon Rainforest showed a decline in biodiversity after deforestation. Which of the following is the most likely cause? A) Increased soil fertility B) Disruption of indigenous plant species C) Introduction of invasive species D) Enhanced water cycle Answer: B) Disruption of indigenous plant species 2. When assessing the sustainability of a new construction project, which factor is most crucial to consider? A) Short-term economic benefits B) Long-term environmental impact C) Immediate availability of materials D) The number of jobs created Answer: B) Long-term environmental impact 3. In a biological study, a marked decrease in fish population in a lake was observed. What research method would best determine the cause? A) Literature review B) Field experiments C) Surveys D) Mathematical modeling