1. Open-ended: Describe the process of DNA replication in eukaryotes, including the roles of key enzymes. Answer: DNA replication in eukaryotes involves the unwinding of the double helix by helicases, followed by the synthesis of a new complementary strand by DNA polymerases. Primase lays down an RNA primer for DNA polymerase to initiate synthesis. The leading strand is synthesized continuously, while the lagging strand is synthesized in Okazaki fragments, which are later joined by DNA ligase. 2. Fill-in-the-blank: The enzyme _______ is responsible for relieving the tension in the DNA helix during replication. Answer: Topoisomerase 3. True/False: Uracil is found in DNA and pairs with adenine. Answer: False. Uracil is found in RNA, not DNA, and it pairs with adenine. 4. Multiple-choice: Which of the following is not a function of nucleic acids? A) Encoding genetic information B) Catalyzing biochemical reactions C) Providing structural support to cells D) Acting as a primary source of energy Answer: D) Acting as a primary source of energy. Nucleic acids are not a primary energy source; that role is filled by molecules like glucose. 5. Open-ended: Explain the significance of the 5' and 3' ends of a nucleic acid strand. Answer: The 5' and 3' ends of a nucleic acid strand refer to the directionality of the molecule, which is critical for processes like DNA replication and transcription. The 5' end has a phosphate