(Some Questions denote their format. All questions are answered) 1. Open-ended: Describe the process of homogenization and its importance in the isolation of enzymes from pig heart tissue. Answer: Homogenization is a process that involves physically breaking down tissues to release cellular contents, including enzymes. It is crucial for disrupting cell membranes and facilitating the extraction of enzymes for further purification. 2. Fill-in-the-blank: The enzyme activity can be measured by monitoring the ________ of the reaction, which is proportional to the enzyme concentration. Answer: Rate Rationale: The rate of an enzymatic reaction is directly related to the enzyme concentration, assuming substrate availability is not limiting. 3. True/False: The presence of protease inhibitors during the isolation process is to prevent the degradation of the target enzyme. Answer: True Rationale: Protease inhibitors are added to protect the enzyme of interest from proteolytic enzymes that can degrade proteins. 4. Multiple-choice: Which of the following is NOT a method used in the purification of enzymes? A) Precipitation B) Dialysis C) Gel electrophoresis D) Centrifugation Answer: C) Gel electrophoresis Rationale: Gel electrophoresis is typically used for analyzing proteins, not for purifying them.