1. True or False: Lactase is effective at breaking down lactose into
glucose and galactose at temperatures above 50°C.
- Answer: False. Rationale: Lactase, like most enzymes, has an
optimal temperature range in which it operates effectively, usually
around 37°C. Temperatures above 50°C can denature the enzyme,
reducing its activity.
2. Fill in the blank: The enzyme lactase has an optimal pH at
which it exhibits peak activity, which is generally around pH
______.
- Answer: 6. Rationale: Lactase's optimal pH is slightly acidic,
reflecting the conditions in the small intestine where it is most
active.
3. Multiple Choice: Which of the following is not a product of
lactase activity?
A) Glucose
B) Galactose
C) Fructose
D) All of the above are products.
- Answer: C) Fructose. Rationale: Lactase specifically catalyzes
the hydrolysis of lactose into glucose and galactose.
4. Open-ended: Describe the mechanism by which lactase
interacts with its substrate.
- Answer: Lactase follows the lock-and-key model where the
lactose molecule fits into the active site of the lactase enzyme.
This binding induces a conformational change in the enzyme,
leading to the cleavage of lactose into glucose and galactose.
5. True or False: Lactase's activity is unaffected by the presence
of competitive inhibitors.
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