(Some Questions denote their format. All questions are answered) 1. True/False: The inhibition of Complex I of the electron transport chain can lead to an increase in reactive oxygen species (ROS) production. - Answer: True. Rationale: Inhibition of Complex I can cause electron leakage and the subsequent reduction of molecular oxygen to form ROS. 2. Fill-in-the-blank: The compound _____ is a well-known inhibitor of Complex III in the electron transport chain. - Answer: Antimycin A. Rationale: Antimycin A binds to the Qi site of Complex III, preventing electron transfer from ubiquinol to cytochrome c. 3. Multiple-choice: Which of the following is not a consequence of electron transport chain inhibition? A) Decreased ATP production B) Increased lactate levels C) Enhanced glycolytic activity D) Elevated levels of NADH - Answer: D) Elevated levels of NADH. Rationale: Inhibition of the electron transport chain would prevent the oxidation of NADH to NAD+, thus decreasing NADH levels. 4. Open-ended: Discuss the potential effects of electron transport chain inhibition on cellular metabolism. - Answer: Inhibition of the electron transport chain can lead to a decrease in ATP production, forcing the cell to rely more on anaerobic metabolism, which is less efficient and results in increased lactate production. This shift can cause a change in the cell's redox state, affecting various metabolic pathways. 5. True/False: Cyanide is an inhibitor that binds irreversibly to the heme group in cytochrome c oxidase.

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