Exam Questions Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogenbase -correctanswer Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: - correct answer Thymine What genes would be screened in a breast cancer panel? - correct answer HER2, ERBB2, BRCA1 Next Generation Sequencing uses: -correctanswer Shortsequence reads Purines and pyrimidines differ from each other in that: - correct answer Purines have two rings; pyrimidines have one ring The purines are: Cytosine and uracil Adenine and thymine Thymineand cytosine Adenineandguanine - correctanswer Adenineandguanine What is the rate of mutation per round of DNA replication? 1 in 1,000 base pairs 1 in 10,000 basepairs 1 in 1,000,000 base pairs 1 in1,000,000,000 base pairs -correct answer 1 in 1,000,000,000 basepairs The rate of DNA migration through an agarose gel during electrophoresis does not dependon which of the following factors? Net charge of the molecule Size of the molecule Shape of the molecule Nucleotide sequence of the molecule - correct answer Nucleotide sequence of the molecule What are the phases in a qPCR Amplification Plot?Initiation, exponential, plateau